Biofilm Services

When it comes to biofilms, our Director Biofilm Research Dr. Matthias T. Buhmann is your perfect partner. The DR. BRILL INSTITUTES group offers a wide range of biofilm studies - with the option of tailoring the experimental conditions to your specific requirements.
We test the efficacy of technologies for the prevention, reduction and control or removal of biofilms for you: from antimicrobial materials, coatings, disinfectants or cleaners.
The variety of methods ranges from the testing of biofilms in wounds or on catheters in the medical field, biofilms on concrete, steel, in car air conditioning systems, or other technical systems such as heat exchangers. Biofilm formation and macrofouling in the maritime sector, e.g. on ships, boats, offshore wind turbines or harbor structures, are also part of our repertoire, as are water-bearing systems for drinking or cooling water.
We have summarized all these topics under DR. BRILL BIOFILM SERVICES for you. Dr. Matthias T. Buhmann will be happy to answer any queries you may have.

Dr. Matthias T. Buhmann Director Biofilm Research
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Medical products containing biocides
Disinfectants and wound dressings

Is your medical device an instrument disinfectant? Please feel free to contact us because we test the efficacy of your disinfectants, for example according to EN 14561/2/3 and EN 17111. You can also have your cleaning products tested by us.

Is your product intended for antimicrobial wound treatment? We can help here too and test according to EN 17854, for example. In addition to the classic standard testing, we have also established other unique procedures on the topic of wounds (e.g. 3D skin model), which you as a customer of Dr. Brill + Dr. Steinmann can benefit from.


Disinfectants
Efficacy testing - biofilms

The fight against biofilms is a major challenge for many manufacturers of biocidal products given regulatory uncertainties on the one hand and technical hurdles on the other. We at Dr. Brill + Dr. Steinmann however, through the development and establishment of various models with regard to biofilms, have a great deal of expertise that you can draw on. Benefit from various technical options that can provide useful insights in the context of biofilms. Among other things, we can offer you the following specific biofilm tests in various bioreactors:

  • "Ring Column" according to DECHEMA
  • "CDC reactor" according to ASTM E2562 in conjunction or supplementation with ASTM E3161 (biofilm cultivation) and ASTM E2871 ("single tube" method)
  • "DFR reactor" according to ASTM E2647
  • "MBEC Assay" according to ASTM A2799
  • Microtiter plate test according to Christensen et al.
  • "Hilpert reactor" according to VDA working group

So if you have a need, we will be happy to inform you in detail. Normally, we have to look at your product and the desired claim and then develop a test model together based on the above-mentioned procedures or something completely new. It is important that the framework of the ECHA Efficacy Guidance is taken into account in order to ensure the regulatory recognition of the procedures. We are ready for this and look forward to your challenges.


Practical testing
Biofilms in drinking water installations

We have an installation that simulates a typical rented apartment in terms of the water supply. Both systems with a central hot water supply and systems with a decentralized hot water supply are integrated into one system. If necessary, we can add disinfection and filtration measures.


Due to the design of the system, water samples can be taken at all relevant points via sampling valves in order to detect planktonic bacteria in the water system. There are also built-in sampling points for biofilms, where we can also expose sample coupons. The system can also be inoculated with specific target organisms, e.g. Legionella or Pseudomonas aeruginosa, to create specific situations.


This system is available for research and testing of antibiofilm measures as part of project work.


Material testing to determine biogenic sulfuric acid corrosion
in mineral materials consisting of mortar or concrete

Biogenic sulfuric acid corrosion (BSA) is a form of damage that occurs in cementitious materials in sewage systems, building drainage systems, sewage treatment plants and biogas plants. Sulfuric acid leads to combined chemical and biological attacks on the surface of the material. The sulfuric acid is the result of various metabolic processes in the biological sulfur cycle, which is controlled by sulfate-reducing (SRB) and sulfur-oxidizing (SOB) bacteria. To expose samples of mineral materials made of concrete or mortar to real-life, accelerated, reproducible BSA, Fraunhofer UMSICHT collaborated with Dr. Brill + Partner GmbH to develop a test rig and procedure that allow test samples to be stored and weathered in a realistic corrosive gas environment (hydrogen sulfide) with SOB typically found in sewage (Thiobacillus). By analyzing and investigating various parameters, the procedure allows conclusions to be drawn regarding the suitability of the material in terms of BSA and suggests optimization potential for the further development of material compounds.

Our services:

  • Production of a defined bacterial inoculum using pure strains cultivated from sewage (Brill)
  • Performance of weathering tests (Fraunhofer)
  • Accompanying microbiological analyses (Brill)
  • Determination of loss of mass and estimate of corrosion rates (Brill, Fraunhofer)
  • 3D scan of sample surface (Fraunhofer)

Efficacy Antifouling
Dr. Brill + Partner Institute for Antifouling and Biocorrosion

In the context of marine applications, the term "biofilm" is only used when it is macroscopically visible. It usually consists of diatoms, small marine algae and juvenile stages of macroalgae. The "braking factor" caused by biofilms on ship hulls can be considerable and increase fuel consumption and carbon dioxide emissions by up to 25%. Therefore, the quantification of such marine biofilms on antifouling coatings before and after cleaning has gained enormous importance. Dr. Brill + Partner Institute for Antifouling and Biocorrosion offers several test methods for this purpose.

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Are you interested in wound healing?
We are as well!

And in order to be a good partner to you in the usual competent manner with regard to this issue, we develop tests for you and with you on the subject of antimicrobial wound treatment products. Since this sector still has some catching up to do in terms of international standards, but we already see a need for action today, we are active on various issues. In particular, the establishment of the concept of biofilms in the context of wound treatment is of great interest, because its special properties must first be understood and taken into account in the treatment. Therefore, we conduct research with our collaboration partners (e.g.: Prof. Dr. med. Ewa Stürmer, UKE; PD Dr. Cornelia Wiegand, Jena University Hospital; Dr. med. Thomas Eberlein, Woundconsulting) on various topics and share our findings on an expert stage:

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New wound infection model based on artificial 3D skin

In the brandnew paper Wiegand et al. 2024 (International Wound Journal, in press) we are presenting parts of our data from our InVitroWound project which was granted by BMBF call "Alternatives to animal testing". In cooperation with PD Dr. Cornelia Wiegand from the University Medical Center Jena we developed an infection wound model based on artificial 3D skin. In this assay we can test antimicrobial wound are products without animals on their antimicrobial, biocompatibility as well as healing performance. This is a milestone in practice-like in-vitro testing with new endpoints.

 

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